本文研究厌氧条件下产电绿脓杆菌P. aeruginosa BTE-1的电化学催化特征。研究结果表明，P. aeruginosa BTE-1菌株在厌氧条件下，不能分泌可充当电子介体的绿脓菌素，但可通过在电极表面形成生物膜呈现了直接电催化性能。P. aeruginosa BTE-1在电极表面形成生物膜与其在特定电极电位下向电极传递电子的过程直接相关，适宜的电位为+0.2 V (vs. SCE)，电位过高可能会损害P. aeruginosa BTE-1细胞。室温范围内升高温度可增强P. aeruginosa BTE-1生物膜电催化活性，但过高的温度(>60℃)会抑制生物膜电催化活性。循环伏安曲线显示，在厌氧条件下形成的P. aeruginosa BTE-1生物膜，具有与典型产电菌株G. sulfurreducens相近的氧化还原电位(-0.4 V~ -0.2 V vs. SCE)。P. aeruginosa BTE-1生物膜可电催化酵母抽取物和葡萄糖，但不能电催化醋酸盐。
周萍
,
张恩仁
,
周立
,
刁国旺
,
牛俊乐
. 绿脓杆菌Pseudomonas aeruginosa BTE-1直接电催化特征[J]. 电化学, 2012
, 18(1)
: 73
-78
.
DOI: 10.61558/2993-074X.2883
The aim of the present study is to investigate the electrocatalytic activity of electricity-producing Pseudomonas aeruginosa BTE-1 strain under anaerobic conditions. Pseudomonas aeruginosa BTE-1 was inoculated into anaerobic three-electrode electrochemical cells, and the electrocatalytic activity was measured at poised potentials. HPLC and cyclic voltammetry were used to detect potential electron mediators in solutions. Experimental results showed that no detectable pyocyanine was excreted by P. aeruginosa BTE-1 strain in the anaerobic electrochemcial cells, and P. aeruginosa BTE-1 exhibited direct electrocatalytic activity through the formation of biofilm on the electrode surface which was induced by the electron transfer from the cells of P. aeruginosa BTE-1 to the electrode at poised potentials. Suitable potential for biofilm formation was found to be 0.2 V (vs. SCE), and more positive potentials would lead to a potential harm to P. aeruginosa BTE-1 cells. At room temperature, the electrocatalytic activity of the P. aeruginosa BTE-1 biofilm could be enhanced by increasing temperature, however, the temperatures higher than 60 °C reduced the electrocatalytic activity of the biofilms quickly. Cyclic voltammetry analysis indicated that P. aeruginosa BTE-1 biofilms formed under anaerobic conditions exhibited an electrochemical catalytic wave in the potential range of -0.4 V to -0.2 V (vs. SCE), similar to that observed with a typical electricity-generating strain, Geobacter sulfurreducens. Organic substrates, such as compounds in yeast and glucose, could be oxidized through the catalysis of P. aeruginosa BTE-1 biofilms, whereas acetate could not be catalyzed to oxidize.
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