胞吐是细胞之间传递信号的主要方式之一,安培法监测细胞胞吐是研究细胞间通讯的基本方法。本研究采用ITO (Indium Tin Oxide) 导电玻璃与紫外光刻技术构建了一种新型ITO微孔电化学传感器,用于细胞胞吐现象的监测并取得较好的效果。相比传统的方法,ITO微孔电化学传感器不仅可以取代传统的碳纤维微电极,而且可与荧光或化学发光等方法联用,运用多种手段同时对细胞的生理现象进行监测。
Exocytosis is one of the major means for cells to transmit messages. Amperometric detection of exocytosis is an important method in studying communication between cells. In this study a new type of ITO micro-pore electrochemical sensor was fabricated by means of ITO and UV light lithographic mask and was used in the detection of exocytosis with promising results. Compared with the conventional method, the ITO micro-pore sensor can not only replace the carbon fiber electrode, but be combined with fluorescent or chemiluminescent methods in detecting the physiological phenomena of cells.
[1] Anselmetti D. Single Cell Analysis Technologies and Applications [M]. Weinheim: WILEY-VCH Verlag GmbH & Co.KGaA, 2009.ⅴ-ⅵ.
[2] Cheng Jie-ke (程介克). Single Cell Analysis (单细胞分析) [M]. Beijing(北京): The Science Press (科学出版社), 2005.ⅲ,383.
[3] Wightman R M, Jankowski J A, Kennedy R T, et al. Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells[J]. Proc. Natl. Acad. Sci. U.S.A. ,1991, 88:10754–10758.
[4] Kawagoe K T, Jankowski J A, Wightman R M. Etched carbon-fiber electrodes as amperometric detectors of catecholamine secretion from isolated biological cells[J]. Anal. Chem. ,1991, 63:1589-1594 .
[5] Chow R H, Von Ruden L, Neher E. Delay in vesicle fusion revealed by electrochemical monitoring of single secretory events in adrenal chromaffin cells [J]. Nature, 1992, 356: 60–63.
[6] Amatore, C, Arbault S, Chen Y et al., Coupling of Electrochemistry and Fluorescence Microscopy at Indium Tin Oxide Microelectrodes for the Analysis of Single Exocytotic Events [J]. Angew. Chem. Int. Ed., 2006, 45: 4000–4003.
[7] Vaughan P F T, Peers C, Walker J H. The use of the human neuroblastoma SH-SY5Y to study the effect of second messenger on noradrenaline release[J]. General Pharmacology , 1995, 26: 1191-1201.
[8] Shi B X, Wang Y, Lam T L, et al. Release monitoring of single cells on a microfluidic device coupled with fluorescence microscopy and electrochemistry [J]. Biomicrofluidics , 2010, 4:1–9.
[9] Andres M I, Forsby A, Walum E. Polygodial-induced noradrenaline release in human neuroblastoma SH-SY5Y cells[J] . Toxicology in Vitro ,1997, 11: 509-511.