葡聚糖及其衍生物对大麦细胞电融合的影响①王伟江志裕＊（复旦大学化学系上海２００４３３）细胞融合是指用人工方法使两个细胞融合成一个细胞的过程［１，２］．体细胞融合可在不同种，不同属，甚至更远的生物间进行，所得到的细胞可具有新的特性．对于植物细胞，有望通...
The influence of dextran and its anion derivates (blue dextran, cibacron blue dexran, fluoresceinythiocarbamoyl dextran) on the electrofusion of barley protoplasts has been investigated. It was found that as a kind of neutral surface active agent, dextran could promote the electrofusion, but these anion derivates could decrease the electrofusion yield. The phenomena were discussed from the view of adsorption of additives on the cell membrane.
1方宗熙．普通遗传学．北京：科学出版社，1984，315～3332李宝键，曾庆平．植物生物技术原理及方法．长沙：湖南科技出版社，1990，302～3203RoosD，DavidsonR，ChoppinP，inSowersA（ed．）．Fusionofcel．NewYork：PlenumPress，19884SongL，BaldwinJ，ReilyR．O，LucyJ．Relationshipbetweensurfaceexposureofacidicphospholipidsandcelfusioninerythrocytessubjectedtoelectricalbreakdown．Biochim．Biophys．Acta，1992，1104：1～85ZhangL，FiedlerU，BergH．Modificationofelectrofusionofbarleyprotoplastsbymembrane－activea－gents．Bioelectrochem．Bioenerg．1991，26：87～966JiangZ，BergH．Increaseofprotoplastelectrofusionsupportedbydextranfractions．Bioelectrochem．bioenerg．，1995，38：383～3877GrobnerU，VelizarorS，BergH．Polylysinesupportselectrofusion．Bioelectrochem．Bioenerg．，1996，39：181～1848BergH．Basicapplicationsofelectricfieldsonbiologicalcel．Bioelectrochem．Bioenerg．，1992，27：89～979ZhangL．Polymer－supportedelectrofusionofbarleyprotoplasts：asynergisticefect．Bioelectrochem．Bioenerg．，1994，34：109～113
