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研究论文

电聚吡咯固定化酪氨酸酶电极的制备及性能

  • 颜流水 ,
  • 魏洽 ,
  • 王承宜 ,
  • 史蓉蓉 ,
  • 马晓通
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  • 南昌航空工业学院环境与化学工程系!江西南昌330034,南昌航空工业学院环境与化学工程系!江西南昌330034,南昌航空工业学院环境与化学工程系!江西南昌330034,南昌航空工业学院环境与化学工程系!江西南昌330034,南昌航空工业学院环境与化学工程系!江西南昌330034

收稿日期: 2000-05-28

  修回日期: 2000-05-28

  网络出版日期: 2000-05-28

Preparation and Characteristics of a Enzyme Electrode Based on Immobilization of Tyrosinase in Electropolymerized Polypyrrole Film

  • YAN Liu_shui ,
  • WEI Qia ,
  • WANG Cheng_Yi ,
  • SHI Rong_rong ,
  • MA Xiao_tong
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  • (Dept. of Environmental and Chem. Eng., Nanchang Institute of Aeronautical Technology, Nanchang 330034, China

Received date: 2000-05-28

  Revised date: 2000-05-28

  Online published: 2000-05-28

摘要

在应用恒电位法电化学聚合吡咯的同时 ,将酪氨酸酶固定在导电聚吡咯膜内 ,制成一种灵敏、稳定的酪氨酸电极 .讨论了溶液 pH值和聚合电位对酶固定化的影响 ,对酶分子嵌入吡咯膜前后的SEM图和CV曲线进行了分析、比较 .该电极对甲苯酚响应的线性范围为 5 .0× 10 -8~ 1.0× 10 -6mol/L ,最适 pH值为 6 .6 ,酶反应表观上遵循Michaelis_Menten动力学 ,表观米氏常数为 2 .2× 10 -5mol/L .

本文引用格式

颜流水 , 魏洽 , 王承宜 , 史蓉蓉 , 马晓通 . 电聚吡咯固定化酪氨酸酶电极的制备及性能[J]. 电化学, 2000 , 6(2) : 222 -226 . DOI: 10.61558/2993-074X.1384

Abstract

A sensitive and stable amperometric enzyme electrode was constructed, based on the immobilization of tyrosinase in potentiostatically electropolymerized polypyrrole film at a platinum electrode. The influence of pH and potential on immbilization was discussed. SEM micrographs and CV curves of films obtained before and after incorparation of tyrosinase molecular were analyzed and compared each other. The enzyme electrode responds linearly to p-cresol over a concentration range of 5×10 -8 ~1×10 -6 mol/L with an optimum pH of 6.6, It exhibited kinetic characteristics of the typical enzyme-catalyzed reaction with an apparent Michaelis constant of 2.2 ×10 -5 mol/L.

参考文献

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