细胞色素c突变体F82H的表面增强拉曼和电化学研究(英文)

郑军伟; 顾仁敖; 陆天虹; George Chumanov

doi:10.61558/2993-074X.3236

电化学 >

2001 , Vol. 7 >Issue 1: 37 - 40

DOI: https://doi.org/10.61558/2993-074X.3236

研究论文

细胞色素c突变体F82H的表面增强拉曼和电化学研究(英文)

郑军伟 ,
顾仁敖 ,
陆天虹 ,
George Chumanov

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苏州大学化学系!江苏苏州215006,苏州大学化学系!江苏苏州215006,中国科学院长春应用化学研究所!吉林长春130022,Dept.of Chem.!Iowa State Univ.,Ames,IA50011,USA,Dept.of Chem.!Iowa State Univ.,Ames,IA50011,USA

收稿日期: 2001-02-28

修回日期: 2001-02-28

网络出版日期: 2001-02-28

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Serrs and Electrochemical Study of Yeast Iso-1-Cytochrome c Mutant F82H

ZHENG Jun?wei ,
GU Re?nao ,
LU Tian?hong ,
George Chumanov

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(1. Dept. of Chem., Suzhou Univ., Suzhou 215006, China; 2. Inst. of Changchun Applied Chem.,Chinese Academy of Sci.,Changchun 130022, China; 3. Dept. o

Received date: 2001-02-28

Revised date: 2001-02-28

Online published: 2001-02-28

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摘要

应用表面增强拉曼光谱和循环伏安法研究了细胞色素c及其突变体F82H的氧化还原性质 .结果表明苯丙氨酸对组氨酸的取代使得蛋白质结构更为稳定 .相对于原体蛋白质 ,突变体的氧化还原电位向负电位方向移动 ,这被归因于由氧化还原过程中伴随有配体转换反应影响所致 .

关键词： 细胞色素c; 突变体; 表面增强拉曼

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郑军伟 , 顾仁敖 , 陆天虹 , George Chumanov . 细胞色素c突变体F82H的表面增强拉曼和电化学研究(英文)[J]. 电化学, 2001 , 7(1) : 37 -40 . DOI: 10.61558/2993-074X.3236

Abstract

The redox properties of iso?1?yeast cytochrome c and its mutant F82H were studied by surface?enhanced Raman spectroscopy and cyclic voltammetry. The results showed that the replacement of phenylalanine?82 with histidine led to a more stable global structure of the protein. A negative shift in the redox potential of the mutant relative to that of wild type protein is ascribed to a ligand switching reaction during the redox processes.

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