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研究论文

长链DNA在金基底上的固定化和电化学标记

  • 周剑章 ,
  • 吴玲玲 ,
  • 董丽琴 ,
  • 林种玉 ,
  • 颜佳伟 ,
  • 董平 ,
  • 包烨
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  • 厦门大学固体表面物理化学国家重点实验室化学系,物理化学研究所!福建厦门361005,厦门大学固体表面物理化学国家重点实验室化学系,物理化学研究所!福建厦门361005,厦门大学固体表面物理化学国家重点实验室化学系,物理化学研究所!福建厦门361005,厦门大学固体表面物理化学国家重

收稿日期: 2001-08-28

  修回日期: 2001-08-28

  网络出版日期: 2001-08-28

Immobilization of Long-Strand DNA onto Gold Surface and Electrochemical Labeling of Hybridization of DNA

  • ZHOU Jian zhang ,
  • WU Ling ling ,
  • DONG Li qin ,
  • LIN Zhong yu ,
  • YAN Jia wei ,
  • DONG Ping ,
  • BAO Yue
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  • (State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China

Received date: 2001-08-28

  Revised date: 2001-08-28

  Online published: 2001-08-28

摘要

本文提出在金基底上用阳离子聚电解质———聚二烯丙基二甲基胺氯化物 (poly(dial lyldimethylammoniumchloride) ,PDDA)自组装膜固定长链DNA的方法 ,用DiffuseReflectanceIn frared ,XPS和STM技术进行表征 ,并对DNA杂交进行电化学标记

本文引用格式

周剑章 , 吴玲玲 , 董丽琴 , 林种玉 , 颜佳伟 , 董平 , 包烨 . 长链DNA在金基底上的固定化和电化学标记[J]. 电化学, 2001 , 7(3) : 276 -280 . DOI: 10.61558/2993-074X.1426

Abstract

Poly(diallyldimethylammonium chloride)(PDDA) was used to facilitate long strand DNA immobilization onto gold surface using self assembly method. Characterization of the PDDA self assembled film and subsequently immobilized DNA were carried out by Diffuse Reflectance Infrared, XPS, STM and electrochemical measurements. Hybridization of DNA was labeled by electrochemical method.

参考文献

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