硝酸还原酶在巯基自组装膜上的电化学性质

宋雅茹; 吴红; 邵会波

doi:10.61558/2993-074X.3302

电化学 >

2002 , Vol. 8 >Issue 3: 333 - 336

DOI: https://doi.org/10.61558/2993-074X.3302

研究论文

硝酸还原酶在巯基自组装膜上的电化学性质

宋雅茹 ,
吴红 ,
邵会波

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北京师范大学化学系,北京师范大学化学系,首都师范大学化学系北京100875 ,北京100875 ,北京100037

收稿日期: 2002-08-28

修回日期: 2002-08-28

网络出版日期: 2002-08-28

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Electrochemical Properties of Nitrate Immobilized on Gold Electrodes with 2-aminoethanethiol SAMs

SONG Ya_ru ,
WU Hong ,
SHAO Hui_bo

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(1.Department of Chemistry,Beijing Normal University,Beijing 100875,China; 2.Department of Chemistry,Capital Normal University,Beijing 100037,China

Received date: 2002-08-28

Revised date: 2002-08-28

Online published: 2002-08-28

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摘要

应用自组装技术将硝酸还原酶 (NR)构筑在硫醇自组装单分子膜上 ,研究了Au/半胱胺 /NR和Au/半胱胺 /NR/卵磷脂两种酶电极在磷酸缓冲液中的直接电化学行为

关键词： 硝酸还原酶; 卵磷脂; 半脘胺自组装膜

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宋雅茹 , 吴红 , 邵会波 . 硝酸还原酶在巯基自组装膜上的电化学性质[J]. 电化学, 2002 , 8(3) : 333 -336 . DOI: 10.61558/2993-074X.3302

Abstract

Nitrate reductase (NR) and phospholipids were immobilized on gold electrodes with 2_aminoethanethio(AET) SAMs. After Au/AET/NR and Au/AET/NR/lipid were soaked in pH 7 phosphate buffers for days,a pair of pre_reversible peak appeared at around 0.202 V and 0.149 V,respsctively, irregularly grew with soaking time in blank buffer.The cathodic peak current(i pcl )of Au/AET/NR system had linear relationship with scan rate between 0.02 and 0.5 V.s -1 ,while i pcl was proportional to V 1/2 in Au/AET/NR/lipid system.The direct electron transfer of enzyme electrodes was strongly affected by pH.Compared with Au/AET,NR exhibited a higher electrochemical activity in Au/AET/lipid system.

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