在谷胱甘肽 (GSH)自组装膜 (SAM )修饰的金电极表面共价固定硫堇 (Th)分子 ,研究其电化学性质及相关应用 ,发现该电极对多巴胺 (DO)有准可逆响应 .利用多巴胺的还原峰可在较高浓度的抗环血酸 (AA)存在条件下 (10 0倍 )对多巴胺进行检测 ,线性响应范围为 1.0～ 30 0 μmol·L- 1.
Thionine was bound onto a glutathione monolayer self_assembled on the surface of a gold electrode previously, the electrochemical characteristics of which were studied. It was found that dopamine had a quasi_reversible response and cathodic characterstics peak could be used to determine the concentration of dopamine in the range of 1.0～300 μmol·L -1 in the presence of a higher concentration of ascorbic acid.
[1] YangSheng_Rong,RenSi_Li,etal.Structureandself_assemblymechanismofself_assembledmonolayers[J].Chem.J .ChineseUniversities,2001,22(3):470.
[2] UlmanA .Formationandstructureofself_assembledmonolayers[J].Chem.Rev.,1996,96:1533.
[3] KontrecJ ,SvetlicicV .Theredoxchangeandphasetransformationinmonolayerfilmsofphenothiazines[J].ElectrochimicaActa,1998,43:389.
[4] RuanChuanmin,YangRu,etal.Areagentlessamperometrichydrogenperoxidebiosensorbasedoncovalent lybindinghorseradishperoxidaseandthionineusingathiol_modifiedgoldelectrode[J].JElectroanalChem,1998,455:121.
[5] OhtaniM ,KuwabataS ,YoneyamaH .Electrochemicaloxidationofreducednicotinamidecoenzymesatauelectrodesmodifiedwithphenothiazinederivativemonolayers[J].JElectroanalChem,1997,422:45.
[6] XuJing_Juan,FangHuai_Qun,ChenHong_Yuan.Theelectrochemicalcharacteristicsofgoldelectrodemodi fiedbythioninecovalentlyboundtoself_assembledcysteaminemonolayeranditselectrocatalyticoxidationforascorbicAcid[J].Chem.J.ChineseUniversities,1997,18(5):706.
