研究GSH在铂、玻碳以及用乙炔黑填充的粉末微电极(AB PME)表面的电化学行为.结果表明,在铂和玻碳电极上,GSH的电化学氧化是一个高度不可逆的反应,相应的CV曲线均未出现氧化电流峰或电流平台.而在AB PME电极上,GSH电化学氧化的表观可逆性得到明显提高,其超电势较常规电极下降约0.5V.且其氧化电流峰,与溶液本体的GSH浓度成良好线性关系.采用此法可完全消除AA和UA对GSH测定的干扰,由"内标法"成功地检测了人体血液中的GSH的浓度.又根据稳态极化曲线和极限扩散电流测定,GSH在AB PME电极表面的电化学氧化是一个单电子反应.

In this paper, the electrochemical behavior of GSH at the surface of platinum(Pt) electrode, glass carbon(GC) electrode and acetylene black packed powder microelectrode (AB-PME) was studied. It was found that the electrochemical oxidations of GSH at both Pt and GC electrode were highly irreversible. No current peak or current plateau could be observed from the CV curves. The direct electro-oxidation of GSH at carbon surface was achieved by using the PME technique.In this case, the apparent reversibility of GSH oxidation was enhanced significatly .The overpotential of GSH oxidation was reduced to 0.5 V at the surface of AB-PME compared with that obtained with Pt and GC electrodes. The exhaustive electrolysis of GSH within the micro-cavity of the PME can be achieved. As a result, the peak currents obtained with AB-PME were proportional to GSH concentration. The interference caused by ascorbic acid and uric acid was eliminated completely by employing AB-PME electrode. The concentration of GSH in human erythrocyte was determined by employing the AB-PME technique and using the method of ”internal standard addition”. The steady state polarization curve of GSH was obtained with the AB-PME electrode .The number involved in the electrochemical oxidation of GSH was calculated as 1 by using the value of I_(L).