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研究论文

酪氨酸酶在硼掺杂金刚石薄膜电极上的固定及酚类化合物的检测

  • 周艳丽 ,
  • 只金芳
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  • 中国科学院理化技术研究所,中国科学院理化技术研究所 北京100080,北京100080

收稿日期: 2006-05-28

  修回日期: 2006-05-28

  网络出版日期: 2006-05-28

Tyrosinase Immobilizarion on Boron-doped Diamond Electrodes and the Determination of Phenolic Compounds

  • ZHOU Yan-li ,
  • ZHI Jin-fang~
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  • (*)(Technical Institute of Physics and Chemistry of CAS,Beijing 100080,China

Received date: 2006-05-28

  Revised date: 2006-05-28

  Online published: 2006-05-28

摘要

以硼掺杂金刚石(boron-doped d iamond,BDD)薄膜作基底,利用光化学反应将含有碳碳双键的烯丙胺化合物修饰在BDD表面,形成氨基单分子层,再经过酰胺键的连接使酪氨酸酶固定在氨基化的金刚石表面,从而制得酪氨酸酶修饰的电极.应用循环伏安法研究该电极用于酚类化合物(包括苯酚、对甲基苯酚和对苯二酚等)检测的灵敏度、线性范围及其稳定性.

本文引用格式

周艳丽 , 只金芳 . 酪氨酸酶在硼掺杂金刚石薄膜电极上的固定及酚类化合物的检测[J]. 电化学, 2006 , 12(2) : 214 -217 . DOI: 10.61558/2993-074X.1725

Abstract

As-grown BDD surfaces were first functionalized by photochemically linking vinyl groups of allylamine,producing covalently linked amine-terminated active BDD surfaces.Then the tyrosinase was immobilized onto the BDD surface through peptide linking.Cyclic voltammograms of phenol,pcresol and hydroquinone were measured using the tyrosinase-modified BDD electrode and amperometric characteristics such as sensitivity,linear response range and stability were studied.

参考文献

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