应用循环伏安法和电化学阻抗谱研究了直流偏压对卵磷脂在玻碳电极表面自组装成膜过程及其结构的影响.实验发现:无论在正偏压还是负偏压条件下,卵磷脂在玻碳电极上均可组装成膜.施加正偏压时,由于玻碳电极表面所带的正电荷与卵磷脂端基之间的静电作用,使得卵磷脂在电极表面倾向于形成双层的类脂膜,并在0.4V偏压下电极阻抗达到最大值.继续增大电极正向偏压,s-BLM缺陷增加,以至趋于被击穿.提出了适宜的等效电路,并据此非线性拟合电极过程,求得部分阻抗的模型参数.研究发现:膜电容和电荷传递电阻呈现良好的互补效应.
李裕辉
,
张洪伟
,
宋桂兰
,
张占军
,
蔡生民
. 直流偏压对于在玻碳电极上双层类脂膜成膜过程的影响[J]. 电化学, 2007
, 13(1)
: 6
-11
.
DOI: 10.61558/2993-074X.1773
The effects of direct current(DC) bias voltage on the structure of lipid membranes forming on a glassy carbon electrode(GCE) was investigated by cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS).The experiment showed that the lipid membranes can be formed with either positive or negative bias potential exerted.When bias voltage was exerted,bilayer lipid membranes tended to form due to the electrostatic interaction between the superficial positive charges on the polarized electrode and the negative charged part of PC.When the positive bias voltage overrun the range,the lacunas on s-BLMs would increase,and the BLMs would be broken.A proper equivalent circuit was nonlinearly fitted,based on the data obtained from the electrochemical process.The result showed that the values of Ret and Cm represented the nicer state of mutual compensation.
[1]Tien HT,Salamon Z.Formation of self-assembled lipidbilayers on solid substrates[J].Bioelectrochem Bioen-erg,1989,22:211.
[2]Salmon Z,Tollin G.Interfacial electrochemistry of cyto-chrome c at a lipid bilayer modified electrode:Effect ofincorporation of negative charges into the bilayer on cy-clic voltammetric parameters[J].BioelectrochemBioenerg,1991,26:321.
[3]Cai H(蔡红),Wang B(王斌),Zhang Z J(张占军),et al.Electrochemical studies and applications of ionicsensor based on the solid supported bilayer lipid mem-brans[J].Chin J Appl Chem(应用化学),2003,4:307.
[4]Cai H(蔡红),Zhang Z J(张占军),Wang B(王斌),et al.Studies of electrochemical sensoral behavior ofhistamine based on the solid supported bilayer lipidmembrane[J].Chin J Chem Phys(化学物理学报),2004,2:206.
[5]Siontorou C G,Oliveira Brett A M,Nikolelis D P.E-valuation of a glassy carbon electrode modified by a bi-layer lipid membrane with incorporated DNA[J].Talan-ta,1996,43:1137.
[6]Castillo J D,Rodriguez A,Roome C A,et al.Lipidfilms as transducers for detection of antigen-antibody andenzyme-substrate reactions[J].Science,1966,153:185. [7]Toro-goyco E,Rodriguez A,Castillo J D.Detection ofanti-insulin antibodies with a new electrical technique:Lipid membrane conductometry[J].Biochem BiophysRes Commun,1996,23:341.
[8]Tong Yuehong,Han Xiaojun,Song Yonghai,et al.Characterization and property of DNAincorporated bilay-er lipid membranes[J].Biophys Chem,2003,105:1. [9]Liu Xiaohua,Huang Weimin,Wang Erkang.An elec-trochemical study on the interaction of surfactin with asupported bilayer lipid membrane on a glassy carbon e-lectrode[J].Electroanal Chem,2005,577:349.
[10]Dimitrios P Nikolelis,Spyros Pantoulias,Ulrich J Krull,et al.Electrochemical transduction of the interactions ofthe sweeteners acesulfame-K,saccharin and cyclamatewith bilayer lipid membranes(BLMs)[J].Electro-chimica Acta,2001,46:1025.
[11]Wang Jianguo,Wang Li,Liu Shaoqin,et al.Interactionof K7Fe3+P2W17O62H2with supported bilayer lipidmembranes on platinum electrode[J].BiophysicalChemistry,2003,106:31.
[12]Diao Peng,Jiang Dianlu,Cui Xiaoli,et al.Cyclic vol-tammetry and a.c.impe dance studies of Ca2+-inducedion channels on Pt-BLM[J].Bioelectrochem Bioenerg,1998,45:173.
[13]Bordi F,Cametti C,Gliozzi A.Impedance measure-ments of self-assembled lipid bilayer membranes on thetip of an electrode[J].Bioelectrochemistry,2002,57:39.
[14]Gerald Wiegand,Noah Arribas-Layton,Heiko Hille-brandt,et al.Electrical properties of supported lipid bi-layer membranes[J].J Phys Chem B,2002,106:4245. [15]Zhang Z J,Huang J,Wu X Z,et al.Impedance spec-tra of the reduction of adrenochrome[J].J ElectrochemSoc,1999,146:256.
[16]Plant A L.Supported hybrid bilayer membranes as rug-ged cell membrane mimics[J].Langmuir,1999,15:5128.
[17]Boubour E,Lennox R B.Insulating properties of self-assembled monolayers monitored by impedance spec-troscopy[J].Langmuir,2000,16:4222.
