应用电化学恒电流共沉积法在医用纯钛基底上制备羟基磷灰石(HA)/胶原(collagen)复合涂层.SEM和XPS等测试表明:复合涂层呈特定有序的纳-微米二级多孔结构,化学组分为HA和胶原的有机-无机复合.借助体外细胞的培养实验观察种植于不同材料表面的细胞贴壁及生长形态,显示电化学共沉积的复合涂层具有比纯HA或纯钛表面更好的生物相容性.
An electrochemical co-deposition technique has been developed to prepare a hydroxyapatite(HA)/collagen composite coating on Ti substrate.The SEM results show that the composite coating exhibits a homogeneous and porous morphology with nano-micro structure.And the XPS results demonstrate that the composite coating is formed by an organic-inorganic hybrization.In vitro cell culture tests reveal that,compared with pure HA and pure Ti,the HA/collagen composite coating exhibit a best biological properties.
[1]Cheng Xiaoliang,Filiaggib Mark,Roscoe Sharon G.Electrochemically assisted co-precipitation of proteinwith calciumphosphate coatings on titaniumalloy[J].Biomaterials,2004,25:5395-5403.
[2]Fan Yuwei,Duan Ke,Wang Rizhi.A composite coatingby electrolysis-induced collagen self-assembly and calci-um phosphate mineralization[J].Biomaterials,2005,26:1623-1632.
[3]Wan Y Z,Hong L,Jia S R,et al.Synthesis andcharacterization of hydroyxyapatite-bacterial cellulosenanocomposites[J].Composites Science and Technolo-gy,2006,66:1825-1832.
[4]Hu Ren,Shi Haiyan,Lin Changjian.Anovel orderednano hydroxyapatite coating electrochemically depositedon titanium substrate[J].Journal of Biomedical Materi-als Research Part A,2007,80(3):687-692.
[5]Xu J L,Khor K A.Chemical analysis of silica dopedhydroxyapatite biomaterials consolidated by a sparkplasma sintering method[J].Journal of Inorganic Bio-chemistry,2007,101:187-195.
[6]Xue Weichang,Hosick Howard L,Bandyopadhyay Am-it,et al.Preparation and cell materials interactions ofplasma sprayed strontium-containing hydroxyapatitecoating[J].Surface&Coatings Technology,2007,201:4685-4693.
