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研究论文

血红蛋白在类生物膜修饰电极上的直接电化学

  • 后雯璟 ,
  • 周琳 ,
  • 吴霞琴 ,
  • 张红霞 ,
  • 谢文 ,
  • 杨柳 ,
  • 陆中庆
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  • 上海师范大学生命与环境科学学院化学系;

收稿日期: 2008-08-28

  修回日期: 2008-08-28

  网络出版日期: 2008-08-28

Direct Electrochemistry of Hemoglobin on the Electrode Modified with DDAB and SWCNT

  • HOU Wen-jing ,
  • ZHOU Lin ,
  • WU Xia-qin ,
  • ZHANG Hong-xia ,
  • XIE Wen ,
  • YANG Liu ,
  • LU Zhong-qing
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  • (Department of Chemistry,Shanghai Normal University,Shanghai 200234,China

Received date: 2008-08-28

  Revised date: 2008-08-28

  Online published: 2008-08-28

摘要

使用掺合单壁碳纳米管(SWCNT)的不溶性表面活性剂双十二烷基二甲基溴化铵(DDAB)修饰玻碳电极,并将血红蛋白(Hb)固定在修饰膜中制得了稳定的固载Hb的修饰电极.循环伏安和交流阻抗测试表明,固定在电极上的Hb是一个受吸附控制的可逆电子传递过程.该氧化还原过程的CV扫描峰电位与溶液pH值成良好的线性关系,斜率39 mV/pH,表明在发生Hb直接电子传递反应的同时伴随有质子参与反应.掺合SWCNT的类生物膜修饰电极较之不掺合SWCNT的修饰电极对氧气的还原具有更好的催化作用.并以SWCNT掺合量为1 mg.mL-1的DDAB修饰电极性能最佳.

本文引用格式

后雯璟 , 周琳 , 吴霞琴 , 张红霞 , 谢文 , 杨柳 , 陆中庆 . 血红蛋白在类生物膜修饰电极上的直接电化学[J]. 电化学, 2008 , 14(3) : 258 -262 . DOI: 10.61558/2993-074X.1902

Abstract

Hemoglobin(Hb) was immobilized onto the surface of glassy carbon electrode with the single-wall carbon nanotubes and surfactant didodecyldimethylamonium bromide(DDAB) modified previously and its electrochemical behaviors have been investigated by cyclic voltammetry.A pair of well-defined redox peaks has been obtained.Cyclic voltammetry and electrochemical impedance spectroscopy(EIS) were also used to study on the effect of different concentration of SWCNT in DDAB.The result shows that the 1 mg/mL is the best choice.The dependence of E0' on solution pH with the slope of 39 mV/pH,indicated that redox of the Hb is a one electron reaction with two protons transfer.The results also showed that the immobilized Hb keeped its electrocatalytic activity toward the reduction of O2.
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