应用循环伏安法研究山奈素在活化玻碳电极上的电化学行为及其与人血清白蛋白(HSA)的相互作用,建立差示脉冲伏安法测定HSA的新方法.在pH4.5的磷酸盐缓冲液(PBS)中,山奈素可与HSA形成超分子复合物,导致前者在0.468V处的氧化电流峰下降,其下降值与HSA浓度在0.05～0.25mg/L范围呈线性关系,检出限为0.008mg/L,可应用于血清样品测定.
林丽清
,
林新华
,
陈敬华
,
黄丽英
,
刘银环
,
李光文
. 山奈素与蛋白质相互作用的电化学行为研究[J]. 电化学, 2010
, 16(2)
: 233
-236
.
DOI: 10.61558/2993-074X.3350
The electrochemical behavior of kaempferide at activated GCE and the interaction of kaempferide with human serum albumin( HSA) were investigated by cyclic voltammetry. A differential pulse voltammetric method has been proposed for the determination of the HSA with kaempferide. In pH 4. 5 phosphate buffer solutions ( PBS) kaempferide can react with HSA to form an electrochemically non-active supermolecular complex at activated GCE,resulting in the decrease of oxidation peak ( at 0. 468 V) without the change of peak potential. The decrease of the peak current is proportional to HSA concentration in the range of 0. 05 ～ 0. 25 mg/L. The limit of the detection is 0. 008 mg/L. This method can be applied to the determination of HSA sample.
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