应用线性扫描技术研究盐酸表阿霉素的电化学行为.结果表明,在pH 6.80的Tris-HCl缓冲溶液中,盐酸表阿霉素有一较灵敏的还原峰,Epc=-0.34 V(vs.SCE).加入牛血清白蛋白(BSA)后,盐酸表阿霉素的还原峰电流明显下降.据此,建立了BSA的电化学测定法.在最佳实验条件下,1.0×10-9～1.0×10-6 mol.L-1范围内,BSA浓度与盐酸表阿霉素的峰电流下降值△ip呈线性关系(R=0.9973),BSA检出限达8.05×10-10 mol.L-1.盐酸表阿霉素与BSA的结合比为1,结合常数β为3.04×106 L.mol-1.
The linear sweep technique was used to study the electrochemical behavior of epirubicin hydrochloride.The results showed that there was a sensitive reduction peak at-0.34 V(vs.SCE) in pH 6.80 Tris-HCl buffer.When the BSA was added,the value of the reduction peak was reduced deeply.Accordingly,a new electrchemical determintation method of BSA was established.Under the optimum conditions,the decrease of the reduction peak currents of epirubicin hydrochloride were proportional to the concentrations of BSA in the range of 1.0×10-9～1.0×10-6 mol·L-1(R=0.9973).The detection limit of the BSA was 8.05×10-10 mol·L-1.And the binding constant β of the epirubicin hydrochloride and BSA was 3.04×106 L·mol-1 with the ratio of 1.
[1]Ren Haiyang(任海洋),Li Zhiyu(李志裕),Song Bing-sheng(宋炳生).The research progress of anthracycline[J].China Pharmacist(中国药师),2002,5(2):115-116.
[2]María Sereno,Antonella Brunello,Alberto Chiappori,etal.Cardiac toxicity:old and new issues in anti-cancerdrugs[J].Clin Transl Oncol,2008,10:35-46.
[3]National Acopoeia Committee(国家药典委员会).ThePeople's Republic of China Pharmacopoeia[M].Bei-jing:Chemical Industry Press(化学工业出版社),2000,807-809.
[4]Liang Yanqiu(梁彦秋),Zang Shuliang(臧树良),ZhaoXue(赵雪).Interaction of ceftazidime with bovine ser-um album[J].Chinese Journal of Spectroscopy Labora-tory(光谱实验室),2009,26(6):1638-1642.
[5]Zhang Guowen,Zhao Nan,Hu Xing,et al.Interaction ofalpinetin with bovine serum albumin:Probing of themechanismand binding site by spectroscopic methods[J].Spectrochimica Acta Part A,2010,76:410-417.
[6]Zhao Xiaohui(赵小辉),Huang Chengzhi(黄承志).Astudy on the interaction of isamin blue with BSA by res-onance light scattering measurements[J].Journal ofSouthwest China Normal University(西南师范大学学报),2006,31(3):70-75.
[7]Peng Zhen(彭贞),Cheng Lehua(程乐华),Lu Guang-han(陆光汉).Electrochemical study of rhodamine Binteracting with bovine serum albumin(BSA)[J].Jour-nal of Analytical Science(分析科学学报),2006,22(3):318-320.
[8]Yang Pin(杨频).Introduction to bioinorganic chemistry[M].Xi'an:Xi'an Jiaotong University Press(西安交通大学出版社),1991.
