脑神经电化学研究
收稿日期: 2021-12-14
修回日期: 2022-01-04
网络出版日期: 2022-01-10
版权
Brain Electrochemistry
Received date: 2021-12-14
Revised date: 2022-01-04
Online published: 2022-01-10
Copyright
大脑是认知、情感等神经活动的物质基础。脑内神经元通过化学信号及电信号相互连接,共同构成动态而复杂的神经信号网络,实现各项神经活动。因此,对于脑神经化学分子的分析与检测有助于揭示神经生理、病理过程中的分子机制,进而发展神经系统疾病的精准诊断及治疗手段。随着各学科的融合与发展,已有多种分析技术在不同层次实现神经分子的检测。其中,电化学分析方法具有高灵敏、高时空分辨等优势,有望在活体层次上精准描述特定神经分子在神经生理或病理过程中的动态变化。本文围绕选择性以及生理兼容性两大关键问题展开,以本课题组最新研究进展为例,系统阐述了电极界面的构筑原则以及电位型检测方法的独特优势,着重介绍了抗坏血酸在神经生理和病理过程中的动态变化规律,并对脑神经电化学分析领域的发展前景进行了展望。
徐聪 , 江迎 , 于萍 , 毛兰群 . 脑神经电化学研究[J]. 电化学, 2022 , 28(3) : 2108551 . DOI: 10.13208/j.electrochem.210855
Brain, as the source of neural activities such as perceptions and emotions, consists of the dynamic and complex networks of neurons that implement brain functions through electrical and chemical interactions. Therefore, analyzing and monitoring neurochemicals in living brain can greatly contribute to uncovering the molecular mechanism in both physiological and pathological processes, and to taking a further step in developing precise medical diagnosis and treatment against brain diseases. Through collaborations across disciplines, a handful of analytical tools have been proven to be befitting in neurochemical measurement, spanning the level of vesicles, cells, and living brains. Among these, electrochemical methods endowed with high sensitivity and spatiotemporal resolution provide a promising way to precisely describe the dynamics of target neurochemicals during various neural activities. In this review, we expand the discussion on strategies to address two key issues of in vivo electrochemical sensing, namely, selectivity and biocompatibility, taking our latest studies as typical examples. We systematically elaborate for the first time the rationale behind engineering electrode/brain interface, as well as the unique advantages of potentiometric sensing methods. In particular, we highlight our recent progress on employing the as-prepared in vivo electrochemical sensors to unravel the molecular mechanism of ascorbate in physiological and pathological processes, aiming to draw a blueprint for the future development of in vivo electrochemical sensing of brain neurochemicals.
| [1] | Bruns D, Jahn R. Real-time measurement of transmitter release from single synaptic vesicles[J]. Nature, 1995, 377(6544):62-65. |
| [2] | Gao R X, Asano S M, Upadhyayula S, Pisarev I, Milkie D E, Liu T L, Singh V, Graves A, Huynh G H, Zhao Y X, Bogovic J, Colonell J, Ott C M, Zugates C, Tappan S, Rodriguez A, Mosaliganti K R, Sheu S H, Pasolli H A, Pang S, Xu C S, Megason S G, Hess H, Lippincott-Schw-artz J, Hantman A, Rubin G M, Kirchhausen T, Saalfeld S, Aso Y, Boydent E S, Betzig E. Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution[J]. Science, 2019, 363(6424):245. |
| [3] | Mohebi A, Pettibone J R, Hamid A A, Wong J M T, Vinson L T, Patriarchi T, Tian, L, Kennedy R T, Berke J D. Dissociable dopamine dynamics for learning and motivation[J]. Nature, 2019, 570(7759):65-70. |
| [4] | Cserep C, Posfai B, Lenart N, Fekete R, Laszlo Z I, Lele Z, Orsolits B, Molnar G, Heindl S, Schwarcz A D, Ujvari K, Kornyei Z, Toth K, Szabadits E, Sperlagh B, Baranyi M, Csiba L, Hortobagyi T, Magloczky Z, Martinecz B, Szabo G, Erdelyi F, Szipocs R, Tamkun M M, Gesierich B, Duering M, Katona I, Liesz A, Tamas G, Denes A. Microglia monitor and protect neuronal function through specialized somatic purinergic junctions[J]. Science, 2020, 367(6477):528. |
| [5] | Phan N, Li X C, Ewing A G. Measuring synaptic vesicles using cellular electrochemistry and nanoscale molecular imaging[J]. Nat. Rev. Chem., 2017, 1(6):0048. |
| [6] | Wu F, Yu P, Mao L Q. Analytical and quantitative in vivo monitoring of brain neurochemistry by electrochemical and imaging approaches[J]. ACS Omega, 2018, 3(10):13267-13274. |
| [7] | Liu Z C, Tian Y. Recent advances in development of devices and probes for sensing and imaging in the brain[J]. Sci. China-Chem., 2021, 64(6):915-931. |
| [8] | Ding C Q, Zhu A W, Tian Y. Functional surface engineering of C-dots for fluorescent biosensing and in vivo bioimaging[J]. Acc. Chem. Res., 2014, 47(1):20-30. |
| [9] | Patriarchi T, Cho J R, Merten K, Howe M W, Marley A, Xiong W H, Folk R W, Broussard G J, Liang R, Jang M J, Zhong H, Dombeck D, von Zastrow M, Nimmerjahn A, Gradinaru V, Williams J T, Tian L. Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors[J]. Science, 2018, 360(6396):1420. |
| [10] | Sun F M, Zeng J Z, Jing M, Zhou J H, Feng J S, Owen S F, Luo Y C, Li F N, Wang H, Yamaguchi T, Yong Z H, Gao Y J, Peng W L, Wang L Z, Zhang S Y, Du J L, Lin D Y, Xu M, Kreitzer A C, Cui G H, Li Y L. A genetically encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish, and mice[J]. Cell, 2018, 174(2):481-496. |
| [11] | Tonnesen J, Inavalli V, Nagerl U V. Super-resolution imaging of the extracellular space in living brain tissue[J]. Cell, 2018, 172(5):1108-1121. |
| [12] | Villette V, Chavarha M, Dimov I K, Bradley J, Pradhan L, Mathieu B, Evans S W, Chamberland S, Shi D Q, Yang R Z, Kim B B, Ayon A, Jalil A, St-Pierre F, Schnitzer M J, Bi G Q, Toth K, Ding J, Dieudonne S, Lin M Z. Ultrafast two-photon imaging of a high-gain voltage indicator in awake behaving mice[J]. Cell, 2019, 179(7):1590-1608. |
| [13] | Robinson D L, Hermans A, Seipel A T, Wightman R M. Monitoring rapid chemical communication in the brain[J]. Chem. Rev., 2008, 108(7):2554-2584. |
| [14] | Wilson G S, Johnson M A. In-vivo electrochemistry: What can we learn about living systems?[J]. Chem. Rev., 2008, 108(7):2462-2481. |
| [15] | Wang W, Zhang S H, Li L M, Wang Z L, Cheng J K, Huang W H. Monitoring of vesicular exocytosis from single cells using micrometer and nanometer-sized electrochemical sensors[J]. Anal. Bioanal. Chem., 2009, 394(1):17-32. |
| [16] | Bucher E S, Wightman R M. Electrochemical analysis of neurotransmitters[J]. Annu. Rev. Anal. Chem., 2015, 8(1):239-261. |
| [17] | Xue Y F, Xiao T F, Jiang Y N, Wu F, Yu P, Mao L Q. Progress of in vivo electrochemical analysis of brain neurochemistry[J]. Chinese J. Anal. Chem., 2019, 47(10):1443-1454. |
| [18] | Song E M, Li J H, Won S M, Bai W B, Rogers J A. Materials for flexible bioelectronic systems as chronic neural interfaces[J]. Nat. Mater., 2020, 19(6):590-603. |
| [19] | Rodeberg N T, Sandberg S G, Johnson J A, Phillips P E M, Wightman R M. Hitchhiker’s guide to voltammetry: acute and chronic electrodes for in vivo fast-scan cyclic voltammetry[J]. ACS Chem. Neurosci., 2017, 8(2):221-234. |
| [20] | Roberts J G, Sombers L A. Fast-scan cyclic voltammetry: chemical sensing in the brain and beyond[J]. Anal. Chem., 2018, 90(1):490-504. |
| [21] | Puthongkham P, Venton B J. Recent advances in fast-scan cyclic voltammetry[J]. Analyst, 2020, 145(4):1087-102. |
| [22] | Heien M, Khan A S, Ariansen J L, Cheer J F, Phillips P E M, Wassum K M, Wightman R M. Real-time measurement of dopamine fluctuations after cocaine in brain of behaving rats[J]. Proc. Natl. Acad. Sci. U.S.A., 2005, 102(29):10023-10028. |
| [23] | Xu J C, Qin G G, Luo F, Wang L N, Zhao R, Li N, Yuan J H, Fang X H. Automated stoichiometry analysis of single-molecule fluorescence imaging traces via deep learning[J]. J. Am. Chem. Soc., 2019, 141(17):6979-6985. |
| [24] | Falk T, Mai D, Bensch R, Cicek O, Abdulkadir A, Marrakchi Y, Boehm A, Deubner J, Jaeckel Z, Seiwald K, Dovzhenko A, Tietz O, Dal Bosco C, Walsh S, Saltukoglu D, Tay T L, Prinz M, Palme K, Simons M, Diester I, Brox T, Ronneberger O. U-Net: deep learning for cell counting, detection, and morphometry[J]. Nat. Methods, 2019, 16(1):67-70. |
| [25] | Wang H D, Rivenson Y, Jin Y Y, Wei Z S, Gao R, Gunaydin H, Bentolila L A, Kural C, Ozcan A. Deep learning enables cross-modality super-resolution in fluorescence microscopy[J]. Nat. Methods, 2019, 16(1):103-110. |
| [26] | Xue Y F, Ji W L, Jiang Y N, Yu P, Mao L Q. Deep learning for voltammetric sensing in a living animal brain[J]. Angew. Chem. Int. Ed., 2021, 60(44):23777-23783. |
| [27] | Zhang M N, Yu P, Mao L Q. Rational design of surface/interface chemistry for quantitative in vivo monitoring of brain chemistry[J]. Acc. Chem. Res., 2012, 45(4):533-543. |
| [28] | Wu F, Yu P, Mao L Q. Bioelectrochemistry for in vivo analysis: Interface engineering toward implantable electrochemical biosensors[J]. Curr. Opin. Electrochem., 2017, 5(1):152-157. |
| [29] | Zhan L M, Tian Y. Designing recognition molecules and tailoring functional surfaces for in vivo monitoring of small molecules in the brain[J]. Acc. Chem. Res., 2018, 51(3):688-696. |
| [30] | Lin Y Q, Liu K, Yu P, Xiang L, Li X C, Mao L Q. A facile electrochemical method for simultaneous and on-line measurements of glucose and lactate in brain microdialysate with prussian blue as the electrocatalyst for reduction of hydrogen peroxide[J]. Anal. Chem., 2007, 79(24):9577-9583. |
| [31] | Lin Y Q, Zhu N N, Yu P, Su L, Mao L Q. Physiologically relevant online electrochemical method for continuous and simultaneous monitoring of striatum glucose and lactate following global cerebral ischemia/reperfusion[J]. Anal. Chem., 2009, 81(6):2067-2074. |
| [32] | Huang P C, Mao J J, Yang L F, Yu P, Mao L Q. Bioelectrochemically active infinite coordination polymer nanoparticles: one-pot synjournal and biosensing property[J]. Chem. - Eur. J., 2011, 17(41):11390-11393. |
| [33] | Zhuang X M, Wang D L, Lin Y Q, Yang L F, Yu P, Jiang W, Mao L Q. Strong interaction between imidazolium-based polycationic polymer and ferricyanide: toward redox potential regulation for selective in vivo electrochemical measurements[J]. Anal. Chem., 2012, 84(4):1900-1906. |
| [34] | Lu X L, Cheng H J, Huang P C, Yang L F, Yu P, Mao L Q. Hybridization of bioelectrochemically functional infinite coordination polymer nanoparticles with carbon nanotubes for highly sensitive and selective in vivo electrochemical monitoring[J]. Anal. Chem., 2013, 85(8):4007-4013. |
| [35] | Ma W J, Jiang Q, Yu P, Yang L F, Mao L Q. Zeolitic imidazolate framework-based electrochemical biosensor for in vivo electrochemical measurements[J]. Anal. Chem., 2013, 85(15):7550-7557. |
| [36] | Lin Y Q, Yu P, Hao J, Wang Y X, Ohsaka T, Mao L Q. Continuous and simultaneous electrochemical measurements of glucose, lactate, and ascorbate in rat brain following brain ischemia[J]. Anal. Chem., 2014, 86(8):3895-3901. |
| [37] | Zhang Z P, Hao J, Xiao T F, Yu P, Mao L Q. Online ele-ctrochemical systems for continuous neurochemical measurements with low-potential mediator-based electroche-mical biosensors as selective detectors[J]. Analyst, 2015, 140(15):5039-5047. |
| [38] | Wang Y X, Mao L. Recent advances in analytical metho-dology for in vivo electrochemistry in mammals[J]. Electroanalysis, 2016, 28(2):265-276. |
| [39] | Xiao T F, Wu F, Hao J, Zhang M N, Yu P, Mao L Q. In vivo analysis with electrochemical sensors and biosensors[J]. Anal. Chem., 2017, 89(1):300-313. |
| [40] | Wei H, Wu F, Yu P, Mao L Q. Advances in electrochemical biosensors for in vivo analysis[J]. Chinese J. Anal. Chem., 2019, 47(10):1466-1479. |
| [41] | Xu C, Wu F, Yu P, Mao L Q. In vivo electrochemical sensors for neurochemicals: recent update[J]. ACS Sens., 2019, 4(12):3102-3118. |
| [42] | Pan C, Wei H, Han Z J, Wu F, Mao L Q. Enzymatic electrochemical biosensors for in situ neurochemical measurement[J]. Curr. Opin. Electrochem., 2020, 19:162-167. |
| [43] | Wu F, Yu P, Yang X T, Han Z J, Wang M, Mao L Q. Exploring ferredoxin-dependent glutamate synthase as an enzymatic bioelectrocatalyst[J]. J. Am. Chem. Soc., 2018, 140(40):12700-12704. |
| [44] | Dunn M R, Jimenez R M, Chaput J C. Analysis of aptamer discovery and technology[J]. Nat. Rev. Chem., 2017, 1(10):0076. |
| [45] | Yu P, He X L, Zhang L, Mao L Q. Dual recognition unit strategy improves the specificity of the Adenosine Triphosphate (ATP) aptamer biosensor for cerebral ATP assay[J]. Anal. Chem., 2015, 87(2):1373-1380. |
| [46] | Hou H F, Jin Y, Wei H, Ji W L, Xue Y F, Hu J B, Zhang M N, Jiang Y, Mao L Q. A generalizable and noncovalent strategy for interfacing aptamers with a microelectrode for the selective sensing of neurotransmitters in vivo[J]. Angew. Chem., Int. Ed., 2020, 59(43):18996-19000. |
| [47] | Zhao X, Wang K Q, Li B, Li C Q, Lin Y Q. Preparation, surface modification and in vivo/single cell electroanalytical application of microelectrode[J]. Prog. Chem., 2017, 29(10):1173-1183. |
| [48] | Hu K K, Liu Y L, Oleinick A, Mirkin M V, Huang W H, Amatore C. Nanoelectrodes for intracellular measurements of reactive oxygen and nitrogen species in single living cells[J]. Curr. Opin. Electrochem., 2020, 22:44-50. |
| [49] | Cheng S W, Zhang L, Zhang M N. In vivo detection of hydrogen sulfide in brain and cell[J]. Electroanalysis, 2021, 33:1-15. |
| [50] | Cheng H J, Li L J, Zhang M N, Jiang Y, Yu P, Ma F R, Mao L Q. Recent advances on in vivo analysis of ascorbic acid in brain functions[J]. Trac-Trends Anal. Chem., 2018, 109:247-259. |
| [51] | Ji W L, Zhang M N, Mao L Q. Recent advances on in vivo electrochemical analysis of Vitamin C in rat brain[J]. Chinese J. Anal. Chem., 2019, 47(10):1559-1571. |
| [52] | Rebec G V, Centore J M, White L K, Alloway K D. Ascorbic-acid and the behavioral-response to haloperidol -implications for the action of antipsychotic-drugs[J]. Science, 1985, 227(4685):438-440. |
| [53] | Rice M E. Ascorbate regulation and its neuroprotective role in the brain[J]. Trends Neurosci., 2000, 23(5):209-216. |
| [54] | Zhang M N, Liu K, Gong K P, Su L, Chen Y, Mao L Q. Continuous on-line monitoring of extracellular ascorbate depletion in the rat striatum induced by global ischemia with carbon nanotube-modified glassy carbon electrode integrated into a thin-layer radial flow cell[J]. Anal. Chem., 2005, 77(19):6234-6242. |
| [55] | Zhang M N, Liu K, Xiang L, Lin Y Q, Su L, Mao L Q. Carbon nanotube-modified carbon fiber microelectrodes for in vivo voltammetric measurement of ascorbic acid in rat brain[J]. Anal. Chem., 2007, 79(17):6559-6565. |
| [56] | Xiang L, Yu P, Hao J, Zhang M N, Zhu L, Dai L M, Mao L Q. Vertically aligned carbon nanotube-sheathed carbon fibers as pristine microelectrodes for selective monitoring of ascorbate in vivo[J]. Anal. Chem., 2014, 86(8):3909-3914. |
| [57] | Xiao T F, Jiang Y N, Ji W L, Mao L Q. Controllable and reproducible sheath of carbon fibers with single-walled carbon nanotubes through electrophoretic deposition for in vivo electrochemical measurements[J]. Anal. Chem., 2018, 90(7):4840-4846. |
| [58] | Cui X J, Li W, Ryabchuk P, Junge K, Beller M. Bridging homogeneous and heterogeneous catalysis by heterogeneous single-metal-site catalysts[J]. Nat. Catal., 2018, 1(6):385-397. |
| [59] | Wang A Q, Li J, Zhang T. Heterogeneous single-atom catalysis[J]. Nat. Rev. Chem., 2018, 2(6):65-81. |
| [60] | Wu F, Pan C, He C T, Han Y H, Ma W J, Wei H, Ji W L, Chen W X, Mao J J, Yu P, Wang D S, Mao L Q, Li Y D. Single-atom Co-N4 Electrocatalyst enabling four-electron oxygen reduction with enhanced hydrogen peroxide tolerance for selective sensing[J]. J. Am. Chem. Soc., 2020, 142(39):16861-16867. |
| [61] | Zhou M, Jiang Y, Wang G, Wu W J, Chen W X, Yu P, Lin Y Q, Mao J J, Mao L Q. Single-atom Ni-N4 provides a robust cellular NO sensor[J]. Nat. Commun., 2020, 11(1):3188. |
| [62] | Hou H F, Mao J J, Han Y H, Wu F, Zhang M N, Wang D S, Mao L Q, Li Y D. Single-atom electrocatalysis: a new approach to in vivo electrochemical biosensing[J]. Sci. China-Chem., 2019, 62(12):1720-1724. |
| [63] | Guo S Y, Yan H L, Wu F, Zhao L J, Yu P, Liu H B, Li Y L, Mao L Q. Graphdiyne as electrode material: tuning electronic state and surface chemistry for improved electrode reactivity[J]. Anal. Chem., 2017, 89(23):13008-13015. |
| [64] | Qi H T, Yu P, Wang Y X, Han G C, Liu H B, Yi Y P, Li Y L, Mao L Q. Graphdiyne oxides as excellent substrate for electroless deposition of Pd clusters with high catalytic activity[J]. J. Am. Chem. Soc., 2015, 137(16):5260-5263. |
| [65] | Yan H L, Yu P, Han G C, Zhang Q H, Gu L P, Yi Y P, Liu H B, Li Y L, Mao L Q. High-yield and damage-free exfoliation of layered Graphdiyne in aqueous phase[J]. Angew. Chem. Int. Ed., 2019, 58(3):746-750. |
| [66] | Yan H L, Guo S Y, Wu F, Yu P, Liu H B, Li Y L, Mao L Q. Carbon atom hybridization matters: ultrafast humidity response of Graphdiyne oxides[J]. Angew. Chem. Int. Ed., 2018, 57(15):3922-3926. |
| [67] | Guo S Y, Yu P, Li W Q, Yi Y P, Wu F, Mao L Q. Electron hopping by interfacing semiconducting Graphdiyne nanosheets and redox molecules for selective electrocatalysis[J]. J. Am. Chem. Soc., 2020, 142(4):2074-2082. |
| [68] | Yu P, He X L, Mao L Q. Tuning interionic interaction for highly selective in vivo analysis[J]. Chem. Soc. Rev., 2015, 44(17):5959-5968. |
| [69] | He X L, Zhang K L, Li T, Jiang Y N, Yu P, Mao L Q. Micrometer-Scale ion current rectification at Polyelectrolyte brush-modified micropipets[J]. J. Am. Chem. Soc., 2017, 139(4):1396-1399. |
| [70] | He X L, Zhang K L, Liu Y, Wu F, Yu P, Mao L Q. Chao-tropic monovalent anion-induced rectification inversion at nanopipettes modified by polyimidazolium brushes[J]. Angew. Chem. Int. Ed., 2018, 57(17):4590-4593. |
| [71] | Zhang K L, He X L, Liu Y, Yu P, Fei J J, Mao L Q. Highly selective cerebral ATP assay based on micrometer scale ion current rectification at Polyimidazolium-modified micropipettes[J]. Anal. Chem., 2017, 89(12):6794-6799. |
| [72] | Zhang K L, Wei H, Xiong T Y, Jiang Y N, Ma W J, Wu F, Yu P, Mao L Q. Micrometer-scale transient ion transport for real-time pH assay in living rat brains[J]. Chem. Sci., 2021, 12(21):7369-7376. |
| [73] | Hefti F, Felix D. Chronoamperometry in vivo - Does it interfere with spontaneous neuronal-activity in the brain[J]. J. Neurosci. Methods, 1983, 7(2):151-156. |
| [74] | Zhao L J, Zheng W, Mao L Q. Recent advances of ion-selective electrode for in vivo analysis in brain neurochemistry[J]. Chinese J. Anal. Chem., 2019, 47(10):1480-1491. |
| [75] | Hao J, Xiao T F, Wu F, Yu P, Mao L Q. High antifouling property of ion-selective membrane: toward in vivo monitoring of pH change in live brain of rats with membrane-coated carbon fiber electrodes[J]. Anal. Chem., 2016, 88(22):11238-11243. |
| [76] | Zhao L J, Jiang Y N, Hao J, Wei H, Zheng W, Mao L Q. Graphdiyne oxide enhances the stability of solid contact-based ionselective electrodes for excellent in vivo analysis[J]. Sci. China-Chem., 2019, 62(10):1414-1420. |
| [77] | Zhao L J, Jiang Y, Wei H, Jiang Y N, Ma W J, Zheng W, Cao A M, Mao L Q. In vivo measurement of calcium ion with solid-state ion-selective electrode by using shelled hollow carbon nanospheres as a transducing layer[J]. Anal. Chem., 2019, 91(7):4421-4428. |
| [78] | Wu F, Yu P, Mao L. Self-powered electrochemical systems as neurochemical sensors: toward self-triggered in vivo analysis of brain chemistry[J]. Chem. Soc. Rev., 2017, 46(10):2692-2704. |
| [79] | Wu F, Su L, Yu P, Mao L Q. Role of organic solvents in immobilizing fungus laccase on single-walled carbon nanotubes for improved current response in direct bioelectrocatalysis[J]. J. Am. Chem. Soc., 2017, 139(4):1565-1574. |
| [80] | Wu F, Cheng H J, Wei H, Xiong T Y, Yu P, Mao L Q. Galvanic redox potentiometry for self-driven in vivo measurement of neurochemical dynamics at open-circuit potential[J]. Anal. Chem., 2018, 90(21):13021-13029. |
| [81] | Yu P, Wei H, Zhong P P, Xue Y F, Wu F, Liu Y, Fei J J, Mao L Q. Single-carbon-fiber-powered microsensor for in vivo neurochemical sensing with high neuronal compatibility[J]. Angew. Chem. Int. Ed., 2020, 59(50):22652-22658. |
| [82] | Yue Q W, Li X C, Wu F, Ji W L, Zhang Y, Yu P, Zhang M N, Ma W J, Wang M, Mao L Q. Unveiling the role of DJ-1 protein in vesicular storage and release of catechola-mine with nano/micro-tip electrodes[J]. Angew. Chem. Int. Ed., 2020, 59(27):11061-11065. |
| [83] | Zhang Z P, Zhao L Z, Lin Y Q, Yu P, Mao L Q. Online electrochemical measurements of Ca2+ and Mg2+ in rat brain based on divalent cation enhancement toward electrocatalytic NADH oxidation[J]. Anal. Chem., 2010, 82(23):9885-9891. |
| [84] | Xiao T F, Wang Y X, Wei H, Yu P, Jiang Y, Mao L Q. Electrochemical monitoring of propagative fluctuation of ascorbate in the live rat brain during spreading depolarization[J]. Angew. Chem. Int. Ed., 2019, 58(20):6616-6619. |
| [85] | Jin J, Ji W L, Li L J, Zhao G, Wu W J, Wei H, Ma F R, Jiang Y, Mao L Q. Electrochemically probing dynamics of ascorbate during cytotoxic edema in living rat brain[J]. J. Am. Chem. Soc., 2020, 142(45):19012-19016. |
| [86] | Wang K, Xiao T F, Yue Q W, Wu F, Yu P, Mao L Q. Selective amperometric recording of endogenous ascorbate secretion from a single rat adrenal chromaffin cell with pretreated carbon fiber microelectrodes[J]. Anal. Chem., 2017, 89(17):9502-9507. |
| [87] | Zhang N, Liu J X, Ma F R, Yu L S, Lin Y Q, Liu K, Mao L Q. Change of extracellular ascorbic acid in the brain cortex following ice water vestibular stimulation: an on-line electrochemical detection coupled with in vivo microdialysis sampling for guinea pigs[J]. Chin. Med. J., 2008, 121(12):1120-1125. |
| [88] | Liu J X, Yu P, Lin Y Q, Zhou N, Li T, Ma F R, Mao L Q. In vivo electrochemical monitoring of the change of cochlear perilymph ascorbate during salicylate-induced tinnitus[J]. Anal. Chem., 2012, 84(12):5433-5438. |
| [89] | Lyu Y, Zhang Y W, Tan L, Ji W L, Yu P, Mao L Q, Zhou F. Continuously monitoring of concentration of extracellular ascorbic acid in spinal cord injury model[J]. Chinese J. Anal. Chem., 2017, 45(11):1595-1599. |
| [90] | Xin Y, Song Y, Xiao T F, Zhang Y H, Li L J, Li T, Zhang K, Liu J X, Ma F R, Mao L Q. In vivo recording of ascorbate and neural excitability in medial vestibular nucleus and hippocampus following ice water vestibular stimulation in rats[J]. Electroanalysis, 2018, 30(7):1287-1292. |
| [91] | Zhang Y W, Hou G J, Ji W L, Rao F, Zhou R B, Gao S, Mao L Q, Zhou F. Persistent oppression and simple decompression both exacerbate spinal cord ascorbate levels[J]. Int. J. Med. Sci., 2020, 17(9):1167-1176. |
| [92] | Wang M L, Song Y, Liu J X, Du Y L, Xiong S, Fan X, Wang J, Zhang Z D, Mao L Q, Ma F R. Role of the caudate-putamen nucleus in sensory gating in induced tinnitus in rats[J]. Neural Regener. Res., 2021, 16(11):2250-2256. |
| [93] | Zhang Y H, Li L J, Li T, Xin Y, Liu J X, Ma F R, Mao L Q. In vivo measurement of the dynamics of norepinephrine in an olfactory bulb following ischemia-induced olfactory dysfunction and its responses to dexamethasone treatment[J]. Analyst, 2018, 143(21):5247-5254. |
| [94] | Du Y L, Liu J X, Jiang Q, Duan Q C, Mao L Q, Ma F R. Paraflocculus plays a role in salicylate-induced tinnitus[J]. Hear. Res., 2017, 353:176-184. |
| [95] | Xiong S, Song Y, Liu J X, Du Y L, Ding Y J, Wei H, Bryan K, Ma F R, Mao L Q. Neuroprotective effects of MK-801 on auditory cortex in salicylate-induced tinnitus: Involvement of neural activity, glutamate and ascorbate[J]. Hear. Res., 2019, 375:44-52. |
| [96] | Fan X, Song Y, Du Y L, Liu J X, Xiong S, Zhao G, Wang M L, Wang J, Ma F R, Mao L Q. Participation of the anterior cingulate cortex in sodium salicylate-induced tinnitus[J]. Otology & Neurotology, 2021, 42(8):E1134-E1142. |
| [97] | Xin Y, Zhang Z P, Yu P, Ma F R, Mao L Q. In vivo ele-ctrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation[J]. Sci. China-Chem., 2013, 56(2):256-261. |
| [98] | Feng T T, Zhang S, Zhang L, Zhang M N. Recent advances on antifouling of microelectrode for in vivo electrochemistry[J]. Chinese J. Anal. Chem., 2019, 47(10):1612-1621. |
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