本文用快速简便的方法制备了铁酞菁/氮掺杂石墨烯（FePc/N-G）修饰电极，用于定量检测半胱氨酸. 采用电化学方法对修饰电极的性能进行了表征，结果表明氮掺杂石墨烯负载铁酞菁修饰电极对半胱氨酸具有更宽的线性响应范围和较高的灵敏度.
In this study, we report a facile method to develop FePc/N-G hybrid modified electrode for the detection of L-cysteine. The electrochemical sensor was characterized by cyclic voltammetry and amperometric response. The sensor exhibited a wider linear range with rapid response which has outperformed pure FePc/GCE, highlighting the important role of nitrogen doped graphene as substrate for improving the electrochemical response property.
[1] Ruiz-Diaz J, Torriero A, Salinas E, et al. Enzymatic rotating biosensor for cysteine and glutathione determination in a FIA system[J]. Talanta, 2006, 68(4): 1343-1352.
[2] Obirai J, Nyokong T. Thiol oxidation at 2-mercaptopyrimidine-appended cobalt phthalocyanine modified glassy carbon electrodes[J]. Journal of Electroanalytical Chemistry, 2007, 600(2): 251-256.
[3] Silva F, Silva M, Lima P, et al. A very low potential electrochemical detection of l-cysteine based on a glassy carbon electrode modified with multi-walled carbon nanotubes/gold nanorods[J]. Biosensors and Bioelectronics, 2013, 50: 202-209.
[4] Stone C, Cardosi M, Davis J. A mechanistic evaluation of the amperometric response of reduced thiols in quinone mediated systems[J]. Analytica Chimica Acta, 2003, 491(2): 203-210.
[5] Pereira-Rodrigues N, Cofre R, Zagal J, et al. Electrocatalytic activity of cobalt phthalocyanine CoPc adsorbed on a graphite electrode for the oxidation of reduced l-glutathione (GSH) and the reduction of its disulfide (GSSG) at physiological pH[J]. Bioelectrochemistry, 2007, 70(1): 147-154.
[6] Hayes J, McLellan L. Glutathione and glutathione-dependent enzymes represent a co-ordinately regulated defence against oxidative stress[J]. Free Radical Research, 1999, 31(4): 273-300.
[7] Baldrianova L, Agrafiotou P, Svancara I, et al. The determination of cysteine at Bi-powder carbon paste electrodes by cathodic stripping voltammetry[J]. Electrochemistry Communications, 2008, 10(6): 918-921.
[8] Bayle C, Siri N, Poinsot V, et al. Analysis of tryptophan and tyrosine in cerebrospinal fluid by capillary electrophoresis and “ball lens” UV-pulsed laser-induced fluorescence detection[J]. Journal of Chromatography A, 2003, 1013(1/2): 123-130.
[9] Lima P, Santos W, Oliveira A, et al. Electrocatalytic activity of 4-nitrophthalonitrile-modified electrode for the L-glutathione detection[J]. Journal of Pharmaceutical and Biomedical Analysis, 2008, 47(4/5): 758-764.
[10] Chwatko G, Bald E. Determination of cysteine in human plasma by high-performance liquid chromatography and ultraviolet detection after pre-column derivatization with 2-chloro-1-methylpyridinium iodide[J]. Talanta, 2000, 52(3): 509-515.
[11] Yuan B, Zeng X, Xu C, et al. Electrochemical modification of graphene oxide bearing different types of oxygen functional species for the electro-catalytic oxidation of reduced glutathione original[J]. Sensors and Actuators B: Chemical, 2013, 184: 15-20.
[12] Pang H, Shi Y, Du J, et al. Porous nickel oxide microflowers synthesized by calcination of coordination microflowers and their applications as glutathione electrochemical sensor and supercapacitors[J]. Electrochimica Acta, 2012, 85: 256-262.
[13] Zagal J, Griveau S, Silva J, et al. Metallophthalocyanine-based molecular materials as catalysts for electrochemical reactions[J]. Coordination Chemistry Reviews, 2010, 254(23/24): 2755-2791.
[14] Zagal J, Griveau S, Santander-Nellia M, et al. Carbon nanotubes and metalloporphyrins and metallophthalocyanines-based materials for electroanalysis[J]. Journal of Porphyrins and Phthalocyanines, 2012, 16(7/8): 713-740.
[15] Griveau S, Gulppi M, Pavez J, et al. Cobalt phthalocyanine-based molecular materials for the electrocatalysis and electroanalysis of 2-mercaptoethanol, 2-mercaptoethanesulfonic acid, reduced glutathione and l-cysteine[J]. Electroanalysis, 2003, 15(9): 779-785.
[16] Tu W, Lei J, Jian G, et al. Noncovalent assembly of Picket-Fence porphyrins on nitrogen-doped carbon nanotubes for highly efficient catalysis and biosensing[J]. Chemistry-A European Journal, 2010, 16(13): 4120-4126.
[17] Griveau S, Gulppi M, Bediouia F, et al. Electropolymerized cobalt macrocomplex-based films for thiols electro-oxidation: Effect of the film formation conditions and the nature of the macrocyclic ligand[J]. Solid State Ionics, 2004, 169(1/4): 59-63.
[18] Lezna R, Juanto S, Zagal J. Spectroelectrochemical studies of tetrasulfonated metallophthalocyanines adsorbed on the basal plane of graphite in the presence of cysteine[J]. Journal of Electroanalytical Chemistry, 1998, 452(2): 221-228.
[19] Ozoemena K, Nyokong T. Comparative electrochemistry and electrocatalytic activities of cobalt, iron and manganese phthalocyanine complexes axially co-ordinated to mercaptopyridine self-assembled monolayer at gold electrodes[J]. Electrochimica Acta, 2006, 51(13): 2669-2677.
