采用孔蛋白（MspA）和双肉豆蔻磷脂酰胆碱（DMPC）在玻碳（GC）基底表面成功构建有仿生特性的纳米通道膜，同时将葡萄糖氧化酶（GOD）修饰于膜上. 使用循环伏安法研究GOD/MspA-DMPC/GC电极的GOD直接电化学过程以及其对氧气和葡萄糖的响应. 研究发现，MspA与DMPC形成的仿生纳米通道膜内，GOD在接近生物体系FAD/FADH标准电位处实现了自身两质子、两电子表面控制的电化学反应. MspA与DMPC的仿生纳米通道膜体系为GOD提供了理想活性环境.
Biomimic membrane with nano-channels, which is made up of porin MspA and 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) is constructed on glassy carbon substrate, and glucose oxidase (GOD) is modified on it. The direct electrochemical reaction and electrocatalytic behavior to oxygen and glucose of GOD on the GOD/MspA-DMPC/GC electreode are expounded by the cyclic voltammetric method. The study shows that GOD immobilized on porin MspA and DMPC biomimic membrane displays direct and surface-controlled electrochemical reaction nearby formal potential (E0′) of the flavoprotein active centre (FAD/FADH), and the electrochemical reaction contains two electrons and two protons exchange in 0.1 mmol?L-1 phosphate buffer solution (PBS) (pH 7.0). Furthermore, it is also discovered that, GOD immobilized on porin MspA and DMPC biomimic membrane possesses an excellent bioelectrocatalytic activity for the reduction of O2 and the oxidation of glucose. That is to say, the biomimic nano-channels membrane formed by porin MspA and DMPC provides an ideal living environment for GOD. So, the GOD/MspA-DMPC/GC electrode can be utilized in biosensor and biofuel cell in the future.
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