酪氨酸酶在硼掺杂金刚石薄膜电极上的固定及酚类化合物的检测

电化学（中英文） ›› 2006, Vol. 12 ›› Issue (2): 214-217.

酪氨酸酶在硼掺杂金刚石薄膜电极上的固定及酚类化合物的检测

周艳丽;只金芳;

中国科学院理化技术研究所,中国科学院理化技术研究所北京100080,北京100080

收稿日期:2006-05-28 修回日期:2006-05-28 出版日期:2006-05-28 发布日期:2006-05-28

Tyrosinase Immobilizarion on Boron-doped Diamond Electrodes and the Determination of Phenolic Compounds

ZHOU Yan-li,ZHI Jin-fang~

(*)(Technical Institute of Physics and Chemistry of CAS,Beijing 100080,China

Received:2006-05-28 Revised:2006-05-28 Published:2006-05-28 Online:2006-05-28

摘要/Abstract

摘要： 以硼掺杂金刚石(boron-doped d iamond,BDD)薄膜作基底,利用光化学反应将含有碳碳双键的烯丙胺化合物修饰在BDD表面,形成氨基单分子层,再经过酰胺键的连接使酪氨酸酶固定在氨基化的金刚石表面,从而制得酪氨酸酶修饰的电极.应用循环伏安法研究该电极用于酚类化合物(包括苯酚、对甲基苯酚和对苯二酚等)检测的灵敏度、线性范围及其稳定性.

关键词: 硼掺杂金刚石薄膜, 烯丙胺, 酪氨酸酶, 酚类化合物

Abstract: As-grown BDD surfaces were first functionalized by photochemically linking vinyl groups of allylamine,producing covalently linked amine-terminated active BDD surfaces.Then the tyrosinase was immobilized onto the BDD surface through peptide linking.Cyclic voltammograms of phenol,pcresol and hydroquinone were measured using the tyrosinase-modified BDD electrode and amperometric characteristics such as sensitivity,linear response range and stability were studied.

Key words: Boron-doped diamond, Allylamine, Tyrosinase, Phenolic compounds

中图分类号:

周艳丽;只金芳;. 酪氨酸酶在硼掺杂金刚石薄膜电极上的固定及酚类化合物的检测[J]. 电化学（中英文）, 2006, 12(2): 214-217.

ZHOU Yan-li,ZHI Jin-fang~. Tyrosinase Immobilizarion on Boron-doped Diamond Electrodes and the Determination of Phenolic Compounds[J]. Journal of Electrochemistry, 2006, 12(2): 214-217.

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https://electrochem.xmu.edu.cn/CN/Y2006/V12/I2/214

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