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Journal of Electrochemistry ›› 2019, Vol. 25 ›› Issue (3): 400-408.doi: 10.13208/j.electrochem.181045

• Special Issue: Electrochemical Analysis and Sensing • Previous Articles     Next Articles

Cysteine and Cystamine Co-Self-Assembled Monolayers for  in Vivo Detection of Ascorbic Acid

ZHANG Yue, FENG Tao-tao, JI Wen-liang, ZHANG Mei-ning*   

  1. Department of Chemistry, Renmin University of China, Beijing 100872, China
  • Received:2018-12-12 Revised:2019-03-19 Online:2019-06-25 Published:2019-03-19
  • Supported by:
    We gratefully acknowledge the financial supports from the National Natural Science Foundation of China (Grant No. 21874152) and Renmin University of China.

Abstract: Self-assembled monolayers (SAMs), which form highly ordered monolayers on the electrode surface through the gold-suffer bond, have attracted much attention in recent years. This stable layer not only can regulate the wettable properties of surface, but also can act as a promoter towards redox-active molecules. Here, we developed a simple and effective method to construct cysteine and cystamine co-self-assembled monolayer on gold microelectrode for  in vivo detection of ascorbic acid (AA). The molar ratio at 1:1 of mixed monolayer has been found the optimum to enhance the electron-transfer kinetics of AA oxidation at low potential (ca. 0.10 V), meanwhile, it could resist the non-specific adsorption of protein at electrode surface. The application of the co-self-assembled monolayer is preliminarily demonstrated for  in vivo detection and the basal level of striatum AA was determined to be 257 ± 30 mmol·L-1 (n = 3). This study offers a general and effective approach for  in vivo electrochemistry with high reliability and simplified procedures.

Key words:  cysteine and cystamine, co-self-assembled monolayer, ascorbic acid, anti-adsorption, in vivo

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