In-situ Measurements of Single-stranded DNA Hybridization  and Cleavage by Quartz Crystal Microbalance

Journal of Electrochemistry ›› 2005, Vol. 11 ›› Issue (1): 32-36.

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In-situ Measurements of Single-stranded DNA Hybridization and Cleavage by Quartz Crystal Microbalance

ZHU Long-zhang, GAO Yu-xiang, SHEN He-bai~*, YANG Yong-tao, YUAN Lian-qun

(Dept. of Chem., Shanghai Normal University, Shanghai 200234, China

Received:2005-02-28 Revised:2005-02-28 Online:2005-02-28 Published:2005-02-28

Abstract

Abstract: The use of quartz crystal microbalance (QCM) for in-situ monitoring the immobilization of single-stranded DNA(oligo-1), marked with mercaptol group at 5′ end, on the surface of gold-filled 7.995 MHz AT-cut quartz crystal by Au-S bond with self-assemble technique is reported. And then the hybridization of ssDNA with the complementary 10-mer ODN(oligo-2) and 8-mer ODN(oligo-3) is described. Moreover, the QCM has been employed to analyze the DNA cleavage by cerium(IV) ions under moderate conditions. The results showed that the QCM, which is capable of sensitive measurement, was able to in-situ investigate immobilization, hybridization and cleavage of ssDNA. And the cerium(IV) ions had no cleavage for the double-stranded DNA, but it could hydrolyze single-stranded DNA. So the hydrolytic cleavage of ssDNA at the specific site could be realized by the protection of the hybridization.

Key words: Quartz crystal microbalance, Qligodeoxynucleotide, Hybridization, Hydrolytic cleavage

CLC Number:

Q503

ZHU Long-zhang, GAO Yu-xiang, SHEN He-bai~*, YANG Yong-tao, YUAN Lian-qun. In-situ Measurements of Single-stranded DNA Hybridization and Cleavage by Quartz Crystal Microbalance[J]. Journal of Electrochemistry, 2005, 11(1): 32-36.

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In-situ Measurements of Single-stranded DNA Hybridization and Cleavage by Quartz Crystal Microbalance

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