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Journal of Electrochemistry ›› 2021, Vol. 27 ›› Issue (2): 168-176.  doi: 10.13208/j.electrochem.201247

Special Issue: “电分析与传感”专题文章

• Special Issue of the Journal of Electrochemistry Celebrating 100 Years of Chemistry at Xiamen University • Previous Articles     Next Articles

Luminol/Sulfamic Acid Electrochemiluminescence and Its Application for Dopamine Detection

Tesfaye Hailemariam Barkae1,2,3, Mohamed Ibrahim Halawa1,4,5, Tadesse Haile Fereja1,6, Shimeles Addisu Kitte1,7, Xian-Gui Ma1,2, Ye-Quan Chen1, Guo-Bao Xu1,*()   

  1. 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin,China
    2. School of Applied chemistry and Engineering, University of Science and Technology of China, Hefei 230026, Anhui, China
    3. Wolkite university, College of Natural & Computational Science, Department of Chemistry, P.O Box 07, Wolkite, Ethiopia
    4. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, 35516, Mansoura, Egypt
    5. College of Chemistry and Environmental Engineering and College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, Guangdong, China
    6. Ambo University, College of Medicine and Health Sciences, Department of Pharmacy, P. O. Box 19, Ambo, Ethiopia
    7. Jimma University, College of Natural Sciences, Department of Chemistry, P. O. Box 378, Jimma, Ethiopia
  • Received:2021-02-02 Revised:2021-03-08 Online:2021-04-28 Published:2021-03-15
  • Contact: Guo-Bao Xu E-mail:guobaoxu@ciac.ac.cn


Herein, sulfamic acid (SA) was utilized, for the first time, to enhance significantly the luminol electrochemiluminescence (ECL). With the SA concentration increased from 0.1 μmol·L-1 to 500 μmol·L-1 the ECL intensity increased proportionally. The developed luminol/SA ECL system was employed to detect dopamine (DA) based on its prominent quenching effect. The Stern-Volmer equation of Io/I= 1+Ksv[DA] could be applied to express well the quenching mechanism of DA in the luminol/SA ECL system. The calibration plot showed that the increase in the DA concentration from 0.5 to 20 μmol·L-1 decreased linearly the ECL intensity with a detection limit of 30 nmol·L-1. The luminol/SA ECL system was successfully carried out for DA detection in urine real sample by employing the standard addition method with the excellent recoveries of 103% ~ 105%. Selectivity of the as-developed ECL system was also investigated by using uric acid, ascorbic acid, sugars and amino acids. The results indicated that the ECL intensities changed negligibly in the presence of other substances.

Key words: electrochemiluminescence, sulfamic acid, luminol, dopamine