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Journal of Electrochemistry ›› 2014, Vol. 20 ›› Issue (6): 521-526.doi: 10.13208/j.electrochem.140437

• Special Issue of Bioelectroanalyical Chemistry (Editor: Professor XIA Xing-hua, Nanjing University) • Previous Articles     Next Articles

Preparation of Surface Molecularly Imprinted Magnetic Nanoparticles for Hemoglobin Sensing

HUANG Chun-fang1,2, YAO Gui-hong1, QIU Jian-ding1*   

  1. 1. Department of Chemistry, Nanchang University, Nanchang 330031, China; 2. School of Chemistry and Chemical Engineering, Jinggangshan University, Ji’an 343009, Jiangxi, China
  • Received:2014-06-03 Revised:2014-07-21 Online:2014-12-28 Published:2014-07-26
  • Contact: QIU Jian-ding E-mail:jdqiu@ncu.edu.cn

Abstract: Surface molecularly imprinted magnetic nanoparticles (NPs) were prepared by employing dopamine (DA) as a functional monomer, hemoglobin (Hb) as a template, H2PtCl6 as an oxidant triggered DA polymerization on the surface of the Fe3O4 NPs. The Hb and the formed platinum nanoparticles (PtNPs) were embedded into the polydopamine (PDA). After removal of the Hb, the surface molecularly imprinted magnetic nanoparticles (imprinted Fe3O4/PDA-PtNPs) were formed, and then simply immobilized on the magnetic glassy carbon electrode (imprinted Fe3O4/PDA-PtNPs/MGCE) for Hb sensing. The imprinted Fe3O4/PDA-PtNPs were demonstrated magnetic spherical shape with good solubility in water and narrow size distribution. The PtNPs embedded in the imprinted coating could effectively promote the conductivity and the mechanical strength of imprinted cavity. In addition, the imprinted Fe3O4/PDA-PtNPs/MGCE could selectively recognize Hb and was used to detect Hb in the range of 0.14 ~ 2.7 μg?mL-1 with a detection limit of 0.05 μg?mL-1 (S/N=3).

Key words: molecularly imprinted polymer, magnetic nanoparticles, dopamine, hemoglobin, electrochemical sensor

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